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Title: Influence of L-arginine during bovine in vitro fertilization
Authors: Guimarães Silva, Thiago Velasco
da Silva, Bruno Baraúna
de Sá, André Luiz Alves
da Costa, Nathalia Nogueira
Sampaio, Rafael Vilar
Cordeiro, Marcela da Silva
Santana, Priscila Di Paula Bessa
Adona, Paulo Roberto
Santos, Simone do Socorro Damasceno
Miranda, Moysés dos Santos
Ohashi, Otávio Mitio
Keywords: Blastocysts;Bos indicus;Bos taurus;Cleavage;Embryo;IVF;Oocytes;Semen;Sperm capacitation;Spermatozoa
Issue Date: Dec-2014
Publisher: NISCAIR-CSIR, India
Abstract: The objective of this work was to evaluate the effect of using L-arginine during in vitro fertilization (IVF) on in vitro embryonic development using Bos taurus and Bos indicus semen. Effect of different concentrations (0, 1, 10 and 50 mM) of L-arginine, added to the IVF medium, was evaluated on the fertilization rate at 18 h post-fertilization (hpf), NO3-/NO2- production during IVF by the Griess colorimetric method (30 hpf), cleavage and blastocyst rates (on Day 2 and Day 7 of culture, respectively) and total blastocyst cell number (Day 7 of culture). The results reveal that the addition of 50 mM L-arginine to IVF medium, with either Bos taurus or Bos indicus spermatozoa, decreased the cleavage rate and blastocyst rate compared to the control group. Other concentrations did not affect embryo production. However, 1 mM L-arginine with Bos indicus semen increased the proportion of hatched blastocysts. These results indicate that high L-arginine concentrations may exhibit toxic effects on bovine gametes during in vitro fertilization.
Page(s): 1159-1164
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections: IJEB Vol.52(12) [December 2014]

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