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|Title:||Tagging STMS markers to Fusarium wilt race-1 resistance in chickpea (Cicer arietinum L.)|
Handique, A K
|Keywords:||Chickpea;Fusarium wilt;Fusarium oxysporum;STMS;Tagging;Linkage map|
|Abstract:||Fusarium wilt in chickpea (Cicer arietinum L.) is caused by fungal pathogen Fusarium oxysporum f. sp. ciceris. Eight races of Fusarium have been reported worldwide. In India, races 1 and 2 are the most predominant and cause widespread damage, resulting up to 70% yield loss. Efforts are on to tag molecular markers to loci responsible for resistance against various races of the pathogen. Here we report tagging of STMS (sequence-tagged microsatellite site) markers with race-1 resistance for Fusarium wilt (foc-1). A linkage map was generated using the scored markers on 82 RILs (recombinant inbred lines) of a cross WR-315 × C-104 along with the phenotypic marker. The most closely linked marker, TA37 was identified at a distance of 0.2 cM from foc-1 in linkage group 2 (LG2). Additionally, 2 more markers TA200 and TR2 were also identified at a distance of 1.0 and 3.0 cM, respectively, flanking the foc-1 locus. Markers TA37, TA200 and TR2 can be used in marker assisted selection, introgression of resistance gene (R-gene) into economically important cultivars and finally to clone the candidate gene for Fusarium-wilt resistance.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.13(3) [July 2014]|
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