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Title: Cytomorphological investigations in colchicine-induced polyploids of Lablab purpureus (L.) Sweet
Authors: Dheer, Meenakshi
Sharma, Ram Avatar
Gupta, Ved Prakas
Punia, Sumer Singh
Keywords: Field bean;Induced polyploids;Meiotic behaviour;Colchicine;Quadrivalents
Issue Date: Jul-2014
Publisher: NISCAIR-CSIR, India
Abstract: Successful induction of autotetraploidy by seedling treatment of colchicine has been achieved in Lablab purpureus (L.) Sweet. Young seedlings were treated with different concentrations of aqueous colchicine (0.2, 0.3, 0.4 & 0.5%) using the cotton-swab method, each for different durations. Morphological and cytological variations were studied for C1 and C2 generations. In C1 generation, 4 autotetraploids were obtained with 0.5% colchicine treatment for 6 and 9 h. The tetraploid plants were morphologically different as compared to diploids. Meiosis was irregular and average number of associations per cell ranged from 1.8±0.83 to 2.2±0.44 among quadrivalents and 24.00±0.00 to 19.6±0.89 in bivalents. Bivalent chromosome associations were observed more frequently than quadrivalent associations. Chiasma was not clearly seen. Seeds (10) from suspected tetraploids were used to raise the next generation to evaluate their stability. Only 5 plants were survived up to maturity and of these, one plant was suspected triploid and another plant monosomic. Thus morphological and cytogenetical changes in the polyploidy of L. purpureus, as a result of colchicine treatment, could not be significantly inherited in next generation and successful induction of stable tetraploids in next generation is a challenge for the researchers.
Page(s): 347-355
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.13(3) [July 2014]

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