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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(1) [January 2009] >
| Title: | Characterization of thermoalkalophilic xylanase isolated from Enterobacter isolates |
| Authors: | Sharma, Anjana
Pujari, Rajesh
Patel, Pratibha |
| Keywords: | Characterization
Enterobacter
Enzyme activity
Thermoalkalophilic
Xylanase |
| Issue Date: | Jan-2009 |
| Publisher: | CSIR |
| Abstract: | Thermoalkalophilic xylanase was isolated from Enterobacter spp. with significant activity. Maximum enzyme activity was observed in isolate BGCC#259 (E. cloacae), i.e., 0.056 IU/mL at 24 h, while the highest biomass was observed at 36 h of incubation. Hydrolysis study revealed complete degradation of xylan to xylose after 12 h of incubation. The molecular mass of the xylanase was ~43 kDa. The enzyme was characterized at varied range of pH and temperature, and it was found that all five Enterobacter isolates had a pH and temperature optima of 8.0 and 80ºC, respectively. At 80ºC, xylanase from isolate BGCC#254 (E. cloacae) retained 100% activity, while BGCC#259 retained more than 90% activity after 24 h. |
| Page(s): | 110-114 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.08(1) [January 2009]
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