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Title: Structure-function annotation and phylogenetic strategy of nifH domain of a cyanobacterium—Chlorogloeopsis sp.
Authors: Lakshmi, P T V
Maheswari, S Uma
Annamalai, A
Keywords: BLAST P
Cyanobacteria
Chlorogloeopsis
NifH domain
Phylogram
Issue Date: Jan-2009
Publisher: CSIR
Abstract: Exploration of the available protein sequences of the Cyanobacterial genera Chlorogloeopsis of Stigonematales from the NCBI database to annotate structural and functional domains through BLOCKS SEARCHER and SWISS PDB VIEWER (SWISSMODEL) revealed eleven different important domains, of which nitrogen fixing domain showed high frequency of occurrence (18.18%), among the sequences explored. Therefore, it was particularly compared with other protein sequences available in the databases in order to obtain similarity and to evolve a phylogram. Similarity search for the NifH/frxC family sequence using BLAST P showed that it is similar to nitrogenase protein of the genera Mastigocladus, Nostoc, Anabaena and other uncultured cyanobacterium. Phylogenetic tree based on the similarities obtained from BLAST P indicates the evolutionary relationships.
Page(s): 46-52
ISSN: 0972-5849
Source: IJBT Vol.08(1) [January 2009]

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