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dc.contributor.authorSharma, Bharat Bhushan-
dc.contributor.authorMustafa, Mohd.-
dc.contributor.authorSharma, Tusha-
dc.contributor.authorBanerjee, Basu Dev-
dc.contributor.authorUrfi, Abdul Jamil-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.description.abstractDNA from molted feathers is being increasingly used for genetic studies on birds. However, the DNA obtained from such non-invasive sources is often not of enough quantity and quality for isolation of new microsatellite markers. The present study examined the potential of shed feathers of near threatened Painted Stork as a source of its DNA for cross-species amplification of microsatellites. Thirty-one shed feathers of varying conditions (‘good’ and ‘deteriorated’) and sizes (‘large’, ‘intermediate’ and ‘small’) collected in a north Indian population were used to isolate DNA by a standard isopropanol method and 11 microsatellite markers already developed in the Wood Stork were screened for amplification. Nine plucked feathers from two dead Painted Storks were also used to compare the DNA yield and amplification success. The DNA yield of feathers varied significantly in relation to the calamus size and condition. Among molted feathers, ‘good’ and ‘large’ samples provided more DNA than ‘deteriorated’ and ‘small’ ones, respectively. ‘Large’ plucked feathers yielded more DNA than ‘large’ molted feathers. DNA was almost degraded in all the samples and ratio of absorbance at 260/280 nm varied from 1.0 to 1.8, indicating impurity in many samples. Independent of DNA yields, all microsatellites were cross-amplified in all kinds of feathers, with >80% success in different feather categories. It is concluded that the shed feathers can be successfully used to isolate DNA in the Painted Stork and for cross-species amplification of microsatellites.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.52(10) [October 2014]en_US
dc.subjectNon-invasive samplingen_US
dc.subjectPainted Storken_US
dc.subjectPopulation geneticsen_US
dc.subjectSimple Sequence Repeatsen_US
dc.subjectShed feathersen_US
dc.subjectWood Storken_US
dc.titleCross-species amplification of microsatellite markers in Mycteria leucocephala Pennant 1769: Molted feathers as successful DNA sourceen_US
Appears in Collections:IJEB Vol.52(10) [October 2014]

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