Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/29369
Title: Development and validation of LC-MS/MS method for simultaneous quantitation of testosterone, trenbolone, salbutamol and taleranol in chicken muscle
Authors: Dahiya, Meenakshi
Dubey, Nidhi
Singh, G N
Keywords: LC-MS/MS;Method development;Validation;Testosterone;Trenbolone;Salbutamol;Taleranol;Chicken muscle
Issue Date: Sep-2014
Publisher: NISCAIR-CSIR, India
Abstract: A comparatively simple, sensitive and rapid analytical method has been developed and validated to determine the residues of testosterone, trenbolone, salbutamol and taleranol in chicken muscle using LC-MS/MS in positive ionization mode. Two main parameters that are focused for the identification of target compound is retention time and ion ratio. m/z at 289 > 97, 270 > 198, 240 > 148 and 322 > 304 have been taken as quantifier ion and m/z at 289 >109, 270 >226, 240 > 166 and 322 > 286 have been taken as qualifier ion for testosterone, trenbolone, salbutamol and taleranol, respectively. The LOD and LOQ are found to be 0.2 ng/kg and 0.4 ng/kg for testosterone and 0.1μg/kg and 0.2 μg/kg trenbolone, salbutamol, taleranol, respectively which is below the MRL value fixed by Export Inspection Council, Ministry of Commerce and Industry, Govt. of India. Considering the risk of misuse of veterinary drugs and importance of their monitoring, the present method has advantages, such as simple and robust extraction procedure, lesser consumption of organic solvent, shorter run time of 5.0 min, high throughput analysis, precise, the ability to quantify residues at low detection limits in complex matrix and screening of multiple drug residues in a single run with high selectivity and accuracy.
Page(s): 1211-1217
URI: http://hdl.handle.net/123456789/29369
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-B Vol.53B(09) [September 2014]

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