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Title: In vitro multiplication of Thunia marshalliana Rcbh. f. through mature seeds and pseudonodes
Authors: Singh, Madhulika
Kumaria, Suman
Tandon, Pramod
Keywords: Coconut water;Mature embryo. protocorm;Pseudonode;Seedling growth
Issue Date: Apr-2014
Publisher: NISCAIR-CSIR, India
Abstract: The present paper describes the germination of mature embryo (~seed), seedling development, and in vitro shoot induction and plant regeneration from pseudonode explants of an endangered ornamental orchid, Thunia marshalliana Rcbh. f. The observations on efficacy of nutrient medium composition on in vitro embryo germination and early seedling development demonstrated that nitrogen in any form induced germination and the presence of both organic and inorganic nitrogen into the medium enhanced both germination and seedling development. The modified MS medium having 10% coconut water was found to be the best for germination. Addition of cytokinin did not favour protocorm formation and seedling development. However, multiple shoots were induced directly from the pseudonode explants cultured on MS medium containing different concentrations of 6-benzyl amino purine (BAP) and kinetin (Kn). Amongst the two cytokinins (BAP & Kn) tested, BAP was more effective for direct shoot induction from the pseudonode explants as compared to Kn. The optimal response was observed at 22.0 µM BAP, which induced 3.67 shoots per explant in 3 wk of culture initiation. These shoots (73%) were rooted on their transfer to MS medium. Both the seedlings and rooted shoots were successfully transplanted to plastic pots filled with cow dung, moss, brick pieces and charcoal mixture in equal ratio.
Page(s): 268-273
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.13(2) [April 2014]

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