Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/29062
Title: Synthesis, antitumor activity and G-quadruplex DNA/ct-DNA binding property of a cationic platinum(II) complex of 2-(4-nitro)-imidazo-[5,6-f][1,10]-phenanthroline
Authors: Luo, Xu-Jian
Qin, Qi-Pin
Li, Yu-Lan
Liu, Yan-Cheng
Liang, Hong
Keywords: Bioinorganic chemistry;Platinum;G-quadruplex DNA;Telomeric DNA;Antitumor activity;DNA binding
Issue Date: Jul-2014
Publisher: NISCAIR-CSIR, India
Abstract: A cationic platinum(II) complex (1), [Pt(4-NOIP)(en)]∙Cl2 (4-NOIP = 2-(4-nitro)-imidazo-[5,6-f][1,10]-phenanthroline, en = 1,2-ethylenediamine), has been synthesized and characterized by IR, UV-vis, ESI-MS and element analysis. The interaction of complex (1) with ct-DNA and human telomeric G-quadruplex DNA [Htel-21] has been investigated by circular dichroism absorption and UV–vis absorption titration as well as fluorescent intercalator displacement assay. The circular dichroism absorption data shows that complex (1) can induce linear human telomeric DNA to transform into antiparallel G-quadruplex structure. The binding contants for complex (1) bound to Htel-21 and ct-DNA are 6.89×105 and 7.53×104 M-1, respectively, indicating that complex (1) interacts with Htel-21 more intensively than ct-DNA. The FID assay suggests that complex (1) intercalates into ct-DNA and Htel-21 quite strongly. CD and UV–vis data together with FID experiments have shown that the complex can intercalate more intensely into Htel-21 and ct-DNA and induce formation and stabilization of antiparallel G-quadruplex. The anticancer activity of the complex have been tested against four different cancer cell lines and normal cell line HL-7702; the complex shows a higher activity than cisplatin and only weak cytotoxicity to HL-7702.
Page(s): 787-792
URI: http://hdl.handle.net/123456789/29062
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.53A(07) [July 2014]

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