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IJEB Vol.47(01) [January 2009] >


Title: Production of peptide antifungal antibiotic and biocontrol activity of Bacillus subtilis
Authors: Kumar, Ajay
Saini, Pragati
Shrivastava, J N
Keywords: Antifungal activity
Bacillus subtilis
Dermatophytes
Optimization
Peptide antibiotic.
Issue Date: Jan-2009
Publisher: CSIR
Abstract: Among different bacterial cultures, a potent Bacillus subtilis MTCC-8114 was isolated from garden soil samples which showed 16 and 14 mm inhibition zones by spot inoculation method and 24 and 22 mm inhibition zones by well agar diffusion method against test fungi i.e. Microsporum fulvum and Trichophyton species. Among four media tested, the maximum growth and antibiotic production was found in trypticase soya broth (TSB) medium at 37º C, pH-7 and 48 h of incubation. The Rf value (0.64) by Thin Layer Chromatography (TLC) technique and UV and FTIR spectral data of the active antifungal compound, indicated that the isolated compound belongs to peptide antifungal antibiotic group. MIC value of antifungal antibiotic was 135 and 145 μg/ml.
Page(s): 57-62
ISSN: 0019-5189
Source:IJEB Vol.47(01) [January 2009]

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