Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/28710
Title: Impact of plant growth regulators (PGRs) on callogenesis and artemisinin content in Artemisia annua L. plants
Authors: Mohammad, Anis
Alam, Pravej
Ahmad, Malik M
Ali, Athar
Ahmad, Javed
Abdin, M Z
Keywords: Artemisia annua L.;Callus;Explants;Plant growth regulators;Regeneration
Issue Date: Jan-2014
Publisher: NISCAIR-CSIR, India
Abstract: Callus cultures were initiated from leaf explants of Artemisia annua L. plants for artemisinin production using Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators (PGRs), viz., BAP, NAA, 2,4-D and TDZ. The combination of all PGRs at different concentrations showed a better response in induction of undifferentiated friable calli in the light phase as compared to the dark phase. The calli of transgenic plants over-expressing hmgr gene yielded a biomass of 0.13 g dw/explant in the light in comparison to 0.06 g dw/explant in the dark, when grown on MS medium supplemented with BAP (1.0 mg L-1) and NAA (2.0 mg L-1). The non-transgenic (untransformed plant) calli, however, yielded a biomass of 0.10 g dw/explant in the light when grown on MS medium supplemented with the same concentrations of BAP and NAA, while yield was 0.06 g dw/explant in the dark in MS medium supplemented with BAP (1.0 mg L-1) and 2, 4-D (1.0 mg L-1). Further, the maximum artemisinin content (0.006% on dw basis) was recorded in both transgenic and non-transgenic calli that were grown on MS medium supplemented with BAP (1.0 mg L-1) and NAA (2.0 mg L-1) under light phase.
Page(s): 26-33
URI: http://hdl.handle.net/123456789/28710
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.13(1) [January 2014]

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