NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(1) [January 2008] >


Title: Comparative efficacy of different DNA extraction methods for PCR-based assay in Tectona grandis L.f.
Authors: Narayanan, C
Dubey, Swapnil
Wali, Syed Arif
Shukla, Nidhi
Kumar, Randhir
Mandal, A K
Ansari, S A
Keywords: DNA extraction
ISSR
CTAB
SDS
Teak
Tectona grandis
Issue Date: Jan-2008
Publisher: CSIR
Abstract: Four extraction methods and sample types were evaluated for yield, quality and suitability of genomic DNA for ISSR (inter simple sequence repeats) marker amplification by PCR in teak (Tectona grandis). Both CTAB (hexadecyltrimetylammonium bromide) and SDS (sodium dodecyl sulfate) procedures extracted large amounts of high purity genomic DNA. Leaves of trees, bud grafts and seedlings yielded better DNA than seeds. Using identical PCR conditions, DNA extraction methods and sample types affected amplifications of ISSR markers with a pattern: seedling > graft > tree leaves. Genomic DNA of seed samples contained high amount of protein and showed no ISSR amplifications, which were overcome by inclusion of proteinase K in SDS based extraction method. In conclusion, DNA extraction method and sample types are very important for reproducible ISSR-based molecular marker analysis in teak.
Page(s): 133-136
ISSN: 0972-5849
Source: IJBT Vol.07(1) [January 2008]

Files in This Item:

File Description SizeFormat
IJBT 7(1) 133-136.pdf315.27 kBAdobe PDFView/Open
 Current Page Visits: 642 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 509514 since 06-Feb-2009  Last updated on 11-Apr-2014Webmaster: nopr@niscair.res.in