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Title: Evaluation of α- and β-glucosidase inhibitory properties of macro-algae using intestinal extracts of marine snail, Thais rudolphi (Lamarck, 1822)
Authors: Pramitha, V S
Lipton, A P
Thangaraj, M
Keywords: Glucosidases
Hypnea musciformis
Macro-algal inhibitors
Rapid assay
Thais rudolphi
Ulva fasciata
Issue Date: Jan-2008
Publisher: CSIR
Abstract: Glucosidase-inhibitory activity of marine macro-algae, Ulva fasciata and Hypnea musciformis in different solvents was detected using enzyme-agar plate method. Eenzyme-agar plate containing α- or β-D-glucosidase was used in combination with substrate-agar plate containing p-nitro phenyl α- and β-D-glucopyranoside (PNPG). Discs impregnated with algal extracts placed on the enzyme agar, followed by pre-incubation up to 2.5 h at 25ºC, resulted in the characteristic inhibitory circle around the inhibitor. Extracts of red algae, H. musciformis had higher glucosidase-inhibition than the extracts of U. fasciata. The intestine of Thais rudolphi contained both α- and β-glucosidase enzymes and their inhibition was comparable with the commercial enzymes, suggesting the possible alternate method for assessing the glucosidase-inhibitory activity of the marine macro-algae.
Page(s): 61-65
ISSN: 0972-5849
Source: IJBT Vol.07(1) [January 2008]

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