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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(1) [January 2008] >
| Title: | Evaluation of α- and β-glucosidase inhibitory properties of macro-algae using intestinal extracts of marine snail, Thais rudolphi (Lamarck, 1822) |
| Authors: | Pramitha, V S
Lipton, A P
Thangaraj, M |
| Keywords: | Glucosidases
Hypnea musciformis
Macro-algal inhibitors
Rapid assay
Thais rudolphi
Ulva fasciata |
| Issue Date: | Jan-2008 |
| Publisher: | CSIR |
| Abstract: | Glucosidase-inhibitory activity of marine macro-algae, Ulva fasciata and Hypnea musciformis in different solvents was detected using enzyme-agar plate method. Eenzyme-agar plate containing α- or β-D-glucosidase was used in combination with substrate-agar plate containing p-nitro phenyl α- and β-D-glucopyranoside (PNPG). Discs impregnated with algal extracts placed on the enzyme agar, followed by pre-incubation up to 2.5 h at 25ºC, resulted in the characteristic inhibitory circle around the inhibitor. Extracts of red algae, H. musciformis had higher glucosidase-inhibition than the extracts of
U. fasciata. The intestine of Thais rudolphi contained both α- and β-glucosidase enzymes and their inhibition was comparable with the commercial enzymes, suggesting the possible alternate method for assessing the glucosidase-inhibitory activity of the marine macro-algae. |
| Page(s): | 61-65 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(1) [January 2008]
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