|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(1) [January 2008] >
| Title: | Prokaryotic expression of a 750 bp capsid region of bovine immunodeficiency virus gag gene and development of a recombinant capsid (p26) protein based immunoassay for seroprevalence studies |
| Authors: | Bhatia, Sandeep
Patil, S S
Sood, Richa
Dubey, Renu
Bhatia, Ashok K
Pattnaik, B
Pradhan, H K |
| Keywords: | BIV
Capsid
ELISA
Gag
p26
Seroprevalence |
| Issue Date: | Jan-2008 |
| Publisher: | CSIR |
| Abstract: | A 750 bp DNA fragment of the gag gene, coding for capsid (p26) protein of bovine immunodeficiency virus (BIV), was cloned in pQE32 vector and expressed as 6 His tagged fusion protein in Escherichia coli. The concentration of affinity purified His tagged capsid protein was 5 mg/mL and its yield was 3.5 g/L of induced culture (E. coli). The recombinant capsid protein of BIV was found to be immunologically reactive with a reference positive serum. Using the purified capsid protein, an indirect ELISA was standardized to test sera of cattle and buffalo for carrying out sero-surveillance of BIV. Of 672 animals tested by capsid based ELISA, 162 were positive and 510 were negative, giving an overall prevalence of 24% in India. In conclusion, the recombinant capsid based indirect ELISA was found suitable to study BIV antibody status. To our knowledge, this is the first seroprevalence study of BIV infection in India. |
| Page(s): | 50-55 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(1) [January 2008]
|
|
