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|Title:||Prokaryotic expression of a 750 bp capsid region of bovine immunodeficiency virus gag gene and development of a recombinant capsid (p26) protein based immunoassay for seroprevalence studies|
Patil, S S
Bhatia, Ashok K
Pradhan, H K
|Abstract:||A 750 bp DNA fragment of the gag gene, coding for capsid (p26) protein of bovine immunodeficiency virus (BIV), was cloned in pQE32 vector and expressed as 6 His tagged fusion protein in Escherichia coli. The concentration of affinity purified His tagged capsid protein was 5 mg/mL and its yield was 3.5 g/L of induced culture (E. coli). The recombinant capsid protein of BIV was found to be immunologically reactive with a reference positive serum. Using the purified capsid protein, an indirect ELISA was standardized to test sera of cattle and buffalo for carrying out sero-surveillance of BIV. Of 672 animals tested by capsid based ELISA, 162 were positive and 510 were negative, giving an overall prevalence of 24% in India. In conclusion, the recombinant capsid based indirect ELISA was found suitable to study BIV antibody status. To our knowledge, this is the first seroprevalence study of BIV infection in India.|
|Appears in Collections:|| IJBT Vol.07(1) [January 2008]|
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