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Title: Prokaryotic expression of a 750 bp capsid region of bovine immunodeficiency virus gag gene and development of a recombinant capsid (p26) protein based immunoassay for seroprevalence studies
Authors: Bhatia, Sandeep
Patil, S S
Sood, Richa
Dubey, Renu
Bhatia, Ashok K
Pattnaik, B
Pradhan, H K
Keywords: BIV;Capsid;ELISA;Gag;p26;Seroprevalence
Issue Date: Jan-2008
Publisher: CSIR
Abstract: A 750 bp DNA fragment of the gag gene, coding for capsid (p26) protein of bovine immunodeficiency virus (BIV), was cloned in pQE32 vector and expressed as 6 His tagged fusion protein in Escherichia coli. The concentration of affinity purified His tagged capsid protein was 5 mg/mL and its yield was 3.5 g/L of induced culture (E. coli). The recombinant capsid protein of BIV was found to be immunologically reactive with a reference positive serum. Using the purified capsid protein, an indirect ELISA was standardized to test sera of cattle and buffalo for carrying out sero-surveillance of BIV. Of 672 animals tested by capsid based ELISA, 162 were positive and 510 were negative, giving an overall prevalence of 24% in India. In conclusion, the recombinant capsid based indirect ELISA was found suitable to study BIV antibody status. To our knowledge, this is the first seroprevalence study of BIV infection in India.
Page(s): 50-55
ISSN: 0972-5849
Appears in Collections: IJBT Vol.07(1) [January 2008]

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