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|Title:||Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design|
Aniba, Mohamed Radhouen
Marzouki, Mohamed Nejib
|Keywords:||Xylanase production;Sclerotinia sclerotiorum S2;Factorial design;Wheat bran;Culture aeration;Phosphate anion effect|
|Abstract:||The improvement of xylanase production by Sclerotinia sclerotiorum S2 using a liquid fermentation culture was investigated. The optimized process was divided into three basic steps: (i) evaluating xylanase inducers using different agricultural residues such as wheat bran, oat bran, orange peel and barley bran at 1% final concentration, and also filter paper. Among these, wheat bran showed the maximum activity (2.5 U/ml) at 12 days post-inoculation; (ii) for optimization, we determined the optimal concentration of inducer, the effect of phosphate anion (K₂HPO₄/KH₂PO₄) and culture aeration using a rotary shaker at 100 and 180 rpm. The optimal conditions for these three factors were determined in an experimental panel using factorial data, in which a mathematical model (Minitab software) was fitted; (iii) The optimized culture medium containing a high level of wheat bran (3%) without K₂HPO₄/KH₂PO₄ and submitted to a high agitation (180 rpm/min) increased the xylanase production from 2.5 U/ml to 4 U/ml (1.6-fold).|
|Appears in Collections:||IJBB Vol.45(6) [December 2008]|
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