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Title: Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design
Authors: Ellouze, Olfa
Fattouch, Sami
Mestiri, Faouzi
Aniba, Mohamed Radhouen
Marzouki, Mohamed Nejib
Keywords: Xylanase production
Sclerotinia sclerotiorum S2
Factorial design
Wheat bran
Culture aeration
Phosphate anion effect
Issue Date: Dec-2008
Publisher: CSIR
Abstract: The improvement of xylanase production by Sclerotinia sclerotiorum S2 using a liquid fermentation culture was investigated. The optimized process was divided into three basic steps: (i) evaluating xylanase inducers using different agricultural residues such as wheat bran, oat bran, orange peel and barley bran at 1% final concentration, and also filter paper. Among these, wheat bran showed the maximum activity (2.5 U/ml) at 12 days post-inoculation; (ii) for optimization, we determined the optimal concentration of inducer, the effect of phosphate anion (K₂HPO₄/KH₂PO₄) and culture aeration using a rotary shaker at 100 and 180 rpm. The optimal conditions for these three factors were determined in an experimental panel using factorial data, in which a mathematical model (Minitab software) was fitted; (iii) The optimized culture medium containing a high level of wheat bran (3%) without K₂HPO₄/KH₂PO₄ and submitted to a high agitation (180 rpm/min) increased the xylanase production from 2.5 U/ml to 4 U/ml (1.6-fold).
Page(s): 404-409
ISSN: 0301-1208
Source:IJBB Vol.45(6) [December 2008]

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