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Title: Effect of Na+ ions on pH-dependent conformational changes in brush border sucrase-isomaltase in mice intestine
Authors: Gupta, Shiffalli
Mahmood, Safrun
Khan, Rizwan Hasan
Mahmood, Akhtar
Keywords: Brush border sucrase;Mice intestine;Alkali metal ions;pH-dependent effects;UV-CD spectral analysis;Fluorescence spectral studies
Issue Date: Dec-2008
Publisher: CSIR
Abstract: Intestinal brush border sucrase-isomaltase (sucrose D-glucosidase E.C., E.C. exhibits pH-dependent stimulatory or inhibitory effects in response to Na⁺ ions. However, whether the enzyme undergoes conformational modulations as a function of pH and in the presence of alkali metal ions is not known. In this paper, we investigated the structural and functional relationship of purified murine sucrase in response to pH and Na⁺ ions using UV-CD fluorescence and spectroscopic studies. Kinetic studies revealed that at pH 5.0, the enzyme activation by Na⁺ ions was V-type, which changed to K-type at pH 7.2, whereas at alkaline pH (8.5), Na⁺ ions inhibited the enzyme activity and inhibition was uncompetitive in nature, affecting both the Km and Vmax components. Far UV-CD spectra of protein at pH 7.2 in the absence and presence of Na⁺ were almost overlapping, suggesting that secondary structure of protein was not affected upon addition of the salt. However, near UV-CD spectra indicated marked alterations in the tertiary structure of protein in presence of 50 mM Na⁺ ions. Increase in pH from 7.2 to 8.5 resulted in a marked rise in fluorescence intensity and red shift in λmax due to tryptophan residues in the enzyme molecule. These findings suggested that alterations in enzyme activity as a function of pH and Na⁺ ions was associated with ionization of key amino acid residues together with structural modifications in the enzyme conformation around neutral or alkaline pH.
Page(s): 399-403
ISSN: 0301-1208
Appears in Collections:IJBB Vol.45(6) [December 2008]

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