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Title: Genotyping by icaA gene based PCR of Staphylococcus strains isolated from bovine mastitis cases
Authors: Kuler, Rajiv N
Isloor, S
Suryanarayana, V V S
Veeregowda, B M
Shridhar, N B
Chandrashekhar, N
Sharada, R
Keywords: Biofilm;ica gene;Mastitis;PCR;Staphylococcus
Issue Date: Oct-2013
Publisher: NISCAIR-CSIR, India
Abstract: For biofilm formation, the polysaccharide intercellular adhesion, synthesized by the products of ica gene cluster, is essential. In the present study, the potential of Staphylococcus aureus isolates (25), S. epidermidis isolates (2) and other Staphylococcal species to form biofilm was detected through amplification of icaA gene by PCR (polymerase chain reaction). Results revealed that, of the 25 isolates of S. aureus, 23 showed amplicon of 1.2 kb; whereas no amplicons were observed in other 2 isolates. Further, the 2 isolates of S. epidermidis showed icaA gene amplification. On the other hand, other Staphylococcal species, such as, S. equorum, S. xylosus, S. sciuri subsp. rodentium. S. intermedius, S. saprophyticus, S. haemolyticus, S. capitis subsp. ureolyticus, S. arlettae S. hyicus, S. sciuri, S. sciuri subsp. coranaticus, S. chromogens and S. caseolyticus, did not show any gene amplification. The study showed high prevalence of icaA gene among mastitis isolates of S. aureus. Thus, the presence of ica locus in most of the S. aureus isolates confirms its role as a virulence factor in the pathogenesis of mastitis.
Page(s): 541-543
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.12(4) [October 2013]

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