NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Chemistry -Section A (IJC-A) >
IJC-A Vol.47A [2008] >
IJC-A Vol.47A(12) [December 2008] >

Title: Surface plasmon resonance based biosensor for label free detection of cholesterol
Authors: Gehlot, Rakhee
Sharma, Kavita
Mathew, Manoth
Kumbhat, Sunita
Issue Date: Dec-2008
Publisher: CSIR
IPC CodeInt. Cl. ⁸ A61B5/145; G01N33/92; H01L31/14
Abstract: A surface plasmon resonance based biosensor has been proposed for a simple, label-free, highly selective and sensitive detection of cholesterol employing the flavoenzyme, cholesterol oxidase, as the sensing element. The immobilization of cholesterol oxidase conjugated with bovine serum albumin on to the gold sensor chip has been accomplished by covalent binding through an activated self-assembled 11-mercaptoundecanoic acid monolayers. The surface morphology of the biosensor has been recorded using atomic force microscopy. Sensitivity of 1.41mº/mg dl⁻¹ of cholesterol has been achieved using the biosensor. The biosensor is highly specific for cholesterol and shows no significant interference from potent interferents such as ascorbic acid and uric acid.
Page(s): 1804-1808
ISSN: 0376-4710
Source: IJC-A Vol.47A(12) [December 2008]

Files in This Item:

File Description SizeFormat
IJCA 47A(12) 1804-1808.pdf247.37 kBAdobe PDFView/Open
 Current Page Visits: 2253 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 162375 since 01-Sep-2015  Last updated on 21-Jun-2016Webmaster: