Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/25163
Title: Triptolide induces apoptosis through extrinsic and intrinsic pathways in human osteosarcoma U2OS cells
Authors: Kwon, Haw-Young
Kim, Kyoung-Sook
An, Hyun-Kyu
Moon, Hyung-In
Kim, Hyun-Jun
Lee, Young-Choon
Keywords: Triptolide;U2OS cell;Apoptosis
Issue Date: Dec-2013
Publisher: NISCAIR-CSIR, India
Abstract: Triptolide, a diterpene derived from Tripterygium wilfordii Hook f., a Chinese medicinal herb, has been reported to inhibit cell proliferation and induce apoptosis in various human cancer cells, but its anticancer effects on human osteosarcoma cells have not yet been elucidated. In this study, we investigated whether triptolide induces apoptosis in human osteosarcoma cells and the underlying molecular mechanisms. We firstly demonstrated that triptolide inhibited cell growth and induced apoptosis in U2OS cells. Western blot analysis showed that the levels of procaspase-8, -9, Bcl-2, Bid and mitochondrial cytochrome c were downregulated in triptolide-treated U2OS cells, whereas the levels of Fas, FasL, Bax, cytosolic cytochrome c, cleaved caspase-3 and cleaved PARP were upregulated. These results suggest that triptolide induces apoptosis in U2OS cells by activating both death receptor and mitochondrial apoptotic pathways.
Page(s): 485-491
URI: http://hdl.handle.net/123456789/25163
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.50(6) [December 2013]

Files in This Item:
File Description SizeFormat 
IJBB 50(6) 485-491.pdf610.3 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.