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Title: DNA binding and cleavage activity of a structurally characterized oxobridged diiron(III) complex
Authors: Biswas, Bhaskar
Mitra, Merry
Pal, Abhijit
Basu, Anirban
Rajalakshmi, Subramaniyam
Mitra, Partha
Aliaga-Alcalde, Nuria
Kumar, Gopinatha Suresh
Nair, Balachandran Unni
Ghosh, Rajarshi
Keywords: Coordination chemistry;Iron;Dinuclear compounds;Oxobridged diiron(III) complex;Crystal structure;DNA binding;DNA cleavage
Issue Date: Dec-2013
Publisher: NISCAIR-CSIR, India
Abstract: The synthesis, crystal structure and variable temperature magnetic measurement of an oxobridged dinuclear Fe(III) complex [Fe2O(phen)4Cl2](NO3)2 (1) [phen =1,10-phenanthroline] is reported. From X-ray diffractometry, it is shown that each Fe(III) in the compound is in distorted octahedral environment. Variable temperature magnetic study reveals strong antiferromagnetic behaviour of (1) with = –104±2 cm-1, p = 0.8% and TIP = 280×10-6 cm-1, fixing g = 2.00. The binding property of the complex with calf thymus DNA has been investigated using absorption and emission studies, circular dichroism spectral investigations, thermal melting and viscosity experiments. The binding constant and the linear Stern-Volmer quenching constant of the complex have been determined to be 1.49×103 M-1 (R = 0.99922 for five points) and 2.02×103 (R = 0.99917 for five points), respectively. Spectroscopic and hydrodynamic investigations reveal a mixed mode involving groove binding along with partial intercalative interaction of (1) with CT-DNA. (1) is found to induce oxidative cleavage of the DNA from its supercoiled to both nicked circular and linear forms in a concentration-dependent manner.
Page(s): 1576-1583
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.52A(12) [December 2013]

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