Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/24003
Title: Effect of immunization with lipid associated polysaccharide antigen and anti CD-2 antibodies on class II MHC expression and cellular immune response in BALB/C mice infected with Leishmania donovani
Authors: Bimal, Sanjiva
Bagchi, A K
Das, V
Sinha, P K
Lal, C S
Ranjan, A
Gupta, A K
Kar, S K
Issue Date: Sep-2001
Publisher: NISCAIR-CSIR, India
Abstract: In a bid to characterize the antigens and immunization mechanisms which may be used to produce a protective response against L donovani, role of lipid associated polysaccharide (LPS) antigen and whole antigen was evaluated. BALB/C mice were immunized with whole or LPS antigen in combination with one of three putative adjuvents (anti CD-2 antibody I FIA I 0.85 % Saline). LPS antigen emulsified in anti CD-2 antibody was found to induce significant antibodies in mice on day 28 against challenge with lethal dose of L donovani. Immunoprophylactic properties of LPS and whole antigen was investigated on day 40 through cytokine elicitation (IL-2), MIF) in culture supernatants of spleen cells, but before that MHC-II expressed on macrophage was studied. The LPS antigen in combination with anti CD-2 antibody was found to be most immuno-reactive inducing higher MHC-II expression on macrophages which was associated with substantial rise in the level of MIF and IL-2. It coincided with decline in antibody titre in 100% mice immunized with LPS antigen while Leishmania injected as whole antigen failed to induce specific macrophage and T-cell response with all the above formulations. We surmise from our data that lipid associated polysaccharide antigen linked to anti CD-2 antibody has potential for eliciting protective immunity against Leishmania.
Page(s): 878-882
URI: http://hdl.handle.net/123456789/24003
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.39(09) [September 2001]

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