Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/23971
Title: Modulation of antigenicity of mycelial antigens during developmental cycle of Karnal Bunt (Tilletia indica) of wheat
Authors: Rai, Geeta
Kumar, Anil
Singh, A
Garg, G K
Issue Date: May-2000
Publisher: NISCAIR-CSIR, India
Abstract: Indirect enzyme linked immunosorbent assays (ELISA)were developed using polyclonal antibodies against soluble cytoplasmic (SCA) and insoluble cell wall antigens (ICWA) for monitoring modulation of mycelial antigens during growth cycle of T. indica. With SCA, continuous decrease in ELISA reactivity was observed in maturing fungus cultures, suggesting that SCA were expressed predominantly during early vegetative phase and their decreasing role was apparent as the fungus matures possibly towards sporogenous mycelium. In case of ICWA, the reaction profile showed an increase up to exponential phase of growth probably due to increase in the cell division and branching of mycelium. But later, ICWA antibody reactivity was decreased which may be due to conversion of mycelial phase to sporogenous phase, a quiescent stage of growth. Characterization of changes in antigenic configuration during developmental cycle of Tilletia indica by these antibodies could prove to be useful in identification of developmentally related and virulence marker(s).
Page(s): 488-492
URI: http://hdl.handle.net/123456789/23971
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.38(05) [May 2000]

Files in This Item:
File Description SizeFormat 
IJEB 38(5) 488-492.pdf1.24 MBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.