Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/23857
Title: Isolation, purification and partial chemical characterization of a lethal factor from common Indian toad (Bulo melanostictus, Schneider) skin extract
Authors: Das, Manika
Dasgupta, S C
Gomes, A
Issue Date: Aug-2001
Publisher: NISCAIR-CSIR, India
Abstract: Indian toad (Bufo melanosticus, Schneider) skin extract (TSE) is pharmacologically potent and probably contains several bioactive compounds [Das et. al., Indian J Pharmacal, 28 (1996) 72). A lethal factor was isolated and purified by neutral alumina column chromatography followed by HPLC. Spectroscopic (UV, IR, FAB-MASS) study indicated that the lethal factor (TSE-LF) was a 254 Da long chain compound with carbonyl, hydroxyl and ester as functional groups. LD50 of TSE-LF was found to be 3.5 mg/kg (iv). Biological study showed that TSE-LF possesses hypotensive, cardiotoxic, neurotoxic activity and produced death by apnoea in experimental animal. Cyproheptadine antagonised TSE-LF induced contraction of isolated smooth muscle indicating involvement of histamine/serotonin receptors. TSE-LF induced neurotoxic action on chick biventer cervices was mediated through Ca2+ ion.
Page(s): 781-785
URI: http://hdl.handle.net/123456789/23857
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.39(08) [August 2001]

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