Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBapat, K N-
dc.contributor.authorYenkatesh, M-
dc.contributor.authorSarma, H D-
dc.contributor.authorPillai, M R A-
dc.contributor.authorSainis, K B-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.description.abstractMyocardial infarction (MI) can be monitored using several protein markers including human cardiac myosin (HCM). Monoclonal antibodies were raised against HCM by hybridoma technique. Antimyosin antibody producing clones were identified by ELISA and monoclonality was established by limiting dilution. The antibodies were purified, isotyped and their cross reactions with myosin from other species were estimated. All the clones showed negligible cross reaction with rabbit myosin, but reacted with bovine skeletal myosin to different extents (40-100%). The most avid anti body Mab 4G4 which also strongly reacted with rat cardiac myosin, was labelled with 125I using different oxidising agent such as iodogen. chloramine-T and lac toperoxidase. More than 95% pure radiolabelled antibody could be obtained by gel filtration. The immunoreactivity was retained. Mab 4G4 was also labelled with 99mTc using stannous tartrate as the reducing agent. Radiolabelling yield was~ 60%, the purity was >95%. Both the radiolabelled preparations were tested for biodistribution in rats- both normal and those with induced MI. Approximately 0.7 % of the injected activity/g was found in the infracted region and the accumulation of activity in the infarcted heart was 1.5 times that in the normal heart. A very high percentage of activity (80%) accumulated in the thyroid. With further optimisation of labelling and use of F(ab')2 fragments, better delineation of the infarct sites may become possible. en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.39(06) [June 2001]en_US
dc.titleRadiolabelled monoclonal antibodies against human cardiac myosinen_US
Appears in Collections:IJEB Vol.39(06) [June 2001]

Files in This Item:
File Description SizeFormat 
IJEB 39(6) 526-532.pdf1.53 MBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.