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IJBB Vol.45(5) [October 2008] >

Title: Kinetics of α-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants
Authors: Ghosh, Kallol K
Verma, Santosh Kumar
Keywords: α-Chymotrypsin
4-Nitrophenyl acetate
Micellar enzymology
Alkyldimethyl ethanolammonium bromide
Issue Date: Oct-2008
Publisher: CSIR
Abstract: The kinetics of α-chymotrypsin (α-CT) catalyzed hydrolysis of 4-nitrophenyl acetate has been studied in aqueous solution of alkyldimethylethanolammonium bromide (cetyl, dodecyl, decyl) surfactants at concentrations below and above their critical micelle concentration. From Michaelis-Menten kinetics, the catalytic rate constant kcat and the Michaelis constant KM have been determined. The bell-shaped profiles of α-CT activity with increasing surfactant concentrations indicate the interaction between the micelle-bound enzyme and substrate.
Page(s): 350-353
ISSN: 0301-1208
Source:IJBB Vol.45(5) [October 2008]

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