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Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.45 [2008] >
IJBB Vol.45(5) [October 2008] >
| Title: | Kinetics of α-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants |
| Authors: | Ghosh, Kallol K
Verma, Santosh Kumar |
| Keywords: | α-Chymotrypsin
4-Nitrophenyl acetate
Hydrolysis
Micellar enzymology
Alkyldimethyl ethanolammonium bromide
Surfactants |
| Issue Date: | Oct-2008 |
| Publisher: | CSIR |
| Abstract: | The kinetics of α-chymotrypsin (α-CT) catalyzed hydrolysis of
4-nitrophenyl acetate has been studied in aqueous solution of
alkyldimethylethanolammonium bromide (cetyl, dodecyl, decyl)
surfactants at concentrations below and above their critical
micelle concentration. From Michaelis-Menten kinetics, the
catalytic rate constant kcat and the Michaelis constant KM have
been determined. The bell-shaped profiles of α-CT activity with
increasing surfactant concentrations indicate the interaction
between the micelle-bound enzyme and substrate. |
| Page(s): | 350-353 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.45(5) [October 2008]
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