Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/2377
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dc.contributor.authorYadav, R S S-
dc.contributor.authorYadav, K D S-
dc.date.accessioned2008-11-06T04:14:05Z-
dc.date.available2008-11-06T04:14:05Z-
dc.date.issued2008-10-
dc.identifier.issn0301-1208-
dc.identifier.urihttp://hdl.handle.net/123456789/2377-
dc.description345-349en_US
dc.description.abstractFive indigenous Aspergillus flavus strains MTCC-2206, 1884, 1883, 1783 and 2456 were screened for the secretion of quercetinase. Fungal strains MTCC-2206, 1884, 1883, and 1783 were found to secrete the quercetinase in the range of 0.24-0.36 enzyme unit/mL of the culture medium, while MTCC-2456 secreted only 0.04 enzyme unit/mL. The enzymatic characteristics of quercetinase were determined. The Km values using quercetin as the substrate were 12.5 μM, 14.0 μM, 12.5 μM and 13.0 μM for the quercetinase produced by MTCC-2206, 1884, 1883 and 1783, respectively. The pH optima for the above enzymes were 6.5, 6.5, 6.0 and 6.0 and temperature optima were 45, 40, 45 and 50ºC, respectively. The partial purification from only one strain MTCC- 2206 was achieved (nearly 3-fold purification).en_US
dc.language.isoen_USen_US
dc.publisherCSIRen_US
dc.sourceIJBB Vol.45(5) [October 2008]en_US
dc.subjectQuercetinaseen_US
dc.subjectAspergillus flavusen_US
dc.subjectDioxygenaseen_US
dc.subjectCopper enzymeen_US
dc.titleEnzymatic characteristics of quercetinases from some indigenous Aspergillus flavus strainsen_US
dc.typeArticleen_US
Appears in Collections:IJBB Vol.45(5) [October 2008]

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