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|Title:||Enzymatic characteristics of quercetinases from some indigenous Aspergillus flavus strains|
|Authors:||Yadav, R S S|
Yadav, K D S
|Keywords:||Quercetinase;Aspergillus flavus;Dioxygenase;Copper enzyme|
|Abstract:||Five indigenous Aspergillus flavus strains MTCC-2206, 1884, 1883, 1783 and 2456 were screened for the secretion of quercetinase. Fungal strains MTCC-2206, 1884, 1883, and 1783 were found to secrete the quercetinase in the range of 0.24-0.36 enzyme unit/mL of the culture medium, while MTCC-2456 secreted only 0.04 enzyme unit/mL. The enzymatic characteristics of quercetinase were determined. The Km values using quercetin as the substrate were 12.5 μM, 14.0 μM, 12.5 μM and 13.0 μM for the quercetinase produced by MTCC-2206, 1884, 1883 and 1783, respectively. The pH optima for the above enzymes were 6.5, 6.5, 6.0 and 6.0 and temperature optima were 45, 40, 45 and 50ºC, respectively. The partial purification from only one strain MTCC- 2206 was achieved (nearly 3-fold purification).|
|Appears in Collections:||IJBB Vol.45(5) [October 2008]|
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