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Title: Isolation, characterization and optimization of antifungal activity of an actinomycete of soil origin
Authors: Augustine, S K
Bhavsar, S P
Baserisalehi, M
Kapadnis, B P
Keywords: Antifungal activity;Screening;Streptomyces sp. PU 23
Issue Date: Sep-2004
Publisher: NISCAIR-CSIR, India
Abstract: About 312 actinomycetes were isolated from soil samples on chitin agar. All these isolates were purified and screened for their antifungal activity against pathogenic fungi. Out of these, 22% of the isolates exhibited activity against fungi. One promising isolate with strong antifun gal activity against pathogenic fungi was selected for further studies. This isolate was from Pune, and was active against both yeasts and molds. Various fermentation parameters were optimized. Based on morphological and biochemical parameters, the isolate was identified as Streptomyces. The correlation of antifungal activity with growth indicated growth dependent production of antimetabolite. Maximum antifungal metabolite production (600 units/ml) was achieved in the late log phase, which remained constant during stationery phase, and it was extra cellular in nature.
Page(s): 928-932
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.42(09) [September 2004]

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