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Title: Molecular cloning, expression and characterization of α-amylase gene from a marine bacterium Pseudoalteromonas sp. MY-1
Authors: Tao, Xueying
Jang, Moon-Sun
Kim, Kyoung-Sook
Keywords: Pseudoalteromonas;α Amylase;Cloning;Characterization
Issue Date: Oct-2008
Publisher: CSIR
Abstract: A gene (amyA) encoding an extracellular α-amylase from a marine bacterium Pseudoalteromonas sp. MY-1 was cloned and expressed in Escherichia coli. It comprised an open-reading-frame of 2,007 base pairs and encoded a protein of 669 amino acids with a predicted molecular weight of 73,770 daltons and a pI of 5.15. The entire amino acid sequence of amyA gene showed 86% similarity to the α-amylase preproprotein from Pseudoalteromonas haloplanktis. It consisted of a signal peptide, α-amylase catalytic domain and an amy C domain. The recombinant amylase was purified to homogeneity and biochemically characterized. The enzyme revealed maximum activity at pH 7.0 and 40ºC. The enzyme hydrolyzed soluble starch and some maltooligosaccharides to several oligosaccharides, and maltose was the common product from different substrates.
Page(s): 305-309
ISSN: 0301-1208
Appears in Collections:IJBB Vol.45(5) [October 2008]

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