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Title: A lethal neurotoxic protein from Indian king cobra (Ophiophagus hannah) venom
Authors: De, Pallabi
Dasgupta, S C
Gomes, A
Issue Date: Dec-2002
Publisher: NISCAIR-CSIR, India
Abstract: A Iethal neurotoxin protein (Toxin CM36) was isolated and purified from the Indian King Cobra (Ophiophagus hannah) venom by CM-Sephadex ion exchange chromatography and HPLC. The purifiedtoxin had a SDS- molecular weight of 15 ± 0.5 kD. The UV absorption spectra of Tox in CM36 showed a peak at 280 nm and an Emax at 343.8 nm, when excited at 280nm fluorescence. Toxin CM36 had an LD50 or 3.5 µg/20 g (iv) in male albino mice. It exhibited neurotoxicity and produced irreversible blockade of isolated chick biventer cervicis and rat phrenic nerve diaphragm. The neurotoxicity was found to be Ca2+ dependent. Toxin CM36 had no significant effect on isolated guineapig heart and auricle. It also had no effect on blood pressure of cat and rat but produced respiratory apnoea in rat and guineapig. Toxin CM36 lacked phospholipase activity.
Page(s): 1359-1364
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.40(12) [December 2002]

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