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|Title:||Staphylococcus warneri BW 94-A new source of lipase|
|Authors:||Walavalkar, G S|
Bapat, M M
|Abstract:||Staphylococcus isolated from a common Indian sweet viz. basundi was tested for its ability to produce lipase. The colorless zone of hydrolysis around the colony grown on Baird Parker agar containing egg yolk produced extracellular lipase. Colony morphology, coagulase production, haemolysis, acid production in carbohydrate medium and enzyme activity studies showed that the organism was Staphylococcus warneri. Growth of S. warneri was obtained after 11hr at 37°C, pH 7.5, while the maximum production of lipase was obtained at 30°C at pH 6.5 after 9hr of incubation. Agitation did not increase lipase production. A sudden fall in the activity of lipase was noted after 11hr. Addition of sucrose which is a growth stimulant for Staphylococcus, did not stimulate production of lipase by these organisms. Also, addition of oleic acid, Tween 80 or ethanol did not stimulate formation of lipase.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.40(11) [November 2002]|
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