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|Title:||Morphactin and cytokinin promote high frequency bulbil formation from leaf explants of Curculigo orchioides grown in shake flask cultures|
|Authors:||Nema, Rajesh K|
Ramawat, K G
|Keywords:||Curculigo orchioides;Bulbil formation;Micropropagation;Morphactin;Cytokinin|
|Abstract:||High frequency bulbil formation from leaf explants of an endangered monocot herb, Curculigo orchioides, grown in shake flask cultures has been achieved. The leaf explants were obtained from in vitro grown plantlets maintained on MS solid medium containing BA (0.1 mg/L) and IBA (0.1 mg/L) for the past 8 years. Inoculum density of one leaf explant (~1 cm²) per two mL liquid medium (50 explants 100 mL⁻¹) was recorded optimal for bulbil formation. Increase in bulbil number per explant was recorded with increase in IBA concentration (from 0.1 mg/L to 1 mg/L) in the medium; level of substituted urea in the medium did not alter this response. Maximal percent explant response, number per explant and yield of bulbils was recorded in the medium supplemented with 1.0 mg/L IBA with 0.1 mg/L substituted urea. The number of bulbils per explant doubled during 4 to 8 wks growth while total yield (fresh biomass) increased several-folds. In IBA+2iP combination, optimal number of bulbils with maximum yield per litre was recorded in the medium supplemented with 1.0 mg/L IBA and 0.1 mg/L 2iP, at 8 wks growth. However, maximum fresh weight of bulbils (1027 g) was recorded in the medium containing 0.1 mg/L IBA and 1.0 mg/L 2iP. In morphactin and BA interaction, optimal number (38/explant), yield (19165/L) and FB (1450 g/L) of bulbils was recorded in the medium containing 1.0 mg/L BA and 0.1 mg/L morphactin followed by 0.01 mg/L and 1 mg/L of morphactin with the same concentration of BA. The use of this synthetic growth regulator in bulbil formation was new and very significant over other treatments. Formation of plantlets from bulbils was 80% and their survival was ~80% in the field conditions. These results will be helpful in developing technology for micropropagation of the plant using bioreactor.|
|Appears in Collections:||IJBT Vol.07(4) [October 2008]|
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