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|Title:||Cloning of pediocin PA-1 and its immunity genes from Pediococcus acidilactici K7 using pAMJ shuttle vector into Lactococcus lactis MG1363|
Halami, Prakash M
|Keywords:||Pediococcus acidilactici;Pediocin PA-1;Electroporation;P170 expression system;Lactococcus lactis|
|Abstract:||The matured pediocin PA-1 encoding gene, pedA and its immunity counterpart, pedB from Pediococcus acidilactici K7 were cloned by PCR technique and ligated into the E. coli-lactic shuttle, protein expression vector pAMJ2008. The recombinant pAMJAB was constructed and sequenced to confirm the intactness of reading frame of the deduced pediocin PA-1 fusion protein. Lactococcus lactis MG1363 was electroporated with the plasmids and the transformation efficiency of ~ 2-3 10³ g⁻¹ was observed. This was further confirmed by plasmid DNA isolation and analysis.|
|Appears in Collections:||IJBT Vol.07(4) [October 2008]|
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