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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(4) [October 2008] >
| Title: | Cloning of pediocin PA-1 and its immunity genes from Pediococcus acidilactici K7 using pAMJ shuttle vector into Lactococcus lactis MG1363 |
| Authors: | Srikanth, Anusha
Halami, Prakash M |
| Keywords: | Pediococcus acidilactici
Pediocin PA-1
Electroporation
P170 expression system
Lactococcus lactis |
| Issue Date: | Oct-2008 |
| Publisher: | CSIR |
| Abstract: | The matured pediocin PA-1 encoding gene, pedA and its immunity counterpart, pedB from Pediococcus acidilactici K7 were cloned by PCR technique and ligated into the E. coli-lactic shuttle, protein expression vector pAMJ2008. The recombinant pAMJAB was constructed and sequenced to confirm the intactness of reading frame of the deduced pediocin PA-1 fusion protein. Lactococcus lactis MG1363 was electroporated with the plasmids and the transformation efficiency of ~ 2-3 10³ g⁻¹ was observed. This was further confirmed by plasmid DNA isolation and analysis. |
| Page(s): | 550-553 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.07(4) [October 2008]
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