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Title: Cloning of pediocin PA-1 and its immunity genes from Pediococcus acidilactici K7 using pAMJ shuttle vector into Lactococcus lactis MG1363
Authors: Srikanth, Anusha
Halami, Prakash M
Keywords: Pediococcus acidilactici;Pediocin PA-1;Electroporation;P170 expression system;Lactococcus lactis
Issue Date: Oct-2008
Publisher: CSIR
Abstract: The matured pediocin PA-1 encoding gene, pedA and its immunity counterpart, pedB from Pediococcus acidilactici K7 were cloned by PCR technique and ligated into the E. coli-lactic shuttle, protein expression vector pAMJ2008. The recombinant pAMJAB was constructed and sequenced to confirm the intactness of reading frame of the deduced pediocin PA-1 fusion protein. Lactococcus lactis MG1363 was electroporated with the plasmids and the transformation efficiency of ~ 2-3  10³ g⁻¹ was observed. This was further confirmed by plasmid DNA isolation and analysis.
Page(s): 550-553
ISSN: 0972-5849
Appears in Collections:IJBT Vol.07(4) [October 2008]

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