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IJBT Vol.07(4) [October 2008] >


Title: Effect of wheat straw powder on enhancement of ligninolytic enzyme activity using Phanerochate chrysosporium
Authors: Hossain, S Masud
Anantharaman, N
Keywords: Enhancement
Ligninolytic enzymes
Optimization
Wheat straw
White-rot fungus
Phanerochate chrysosporium
Issue Date: Oct-2008
Publisher: CSIR
Abstract: A comparison made between the suspension culture of white-rot fungus, Phanerochate chrysosporium supplemented with wheat straw powder and control conditions (without wheat straw), revealed an enhancement in the ligninolytic enzymes activity in liquid shake flask in the former. Addition of 2% w/v of wheat straw powder improved greater stability to the lignin peroxidase (Li P) and manganese peroxidase (Mn P) enzymes when compared with the control. At optimum pH (4.5) and optimum temperature (40ºC), maximum enzymatic activity was observed. The optimum shaking speed was found to be 60 rpm. The maximum activity of 538 and 512 μ mol/min mL was obtained for Li P and Mn P with wheat straw powder by the white-rot fungus at optimum conditions, respectively. However, maximum activity of 239 and 215 μ mol/min mL, respectively was obtained for Li P and Mn P at similar conditions in the control.
Page(s): 502-507
ISSN: 0972-5849
Source:IJBT Vol.07(4) [October 2008]

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