Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/2353
Title: Process optimization for citric acid production from raw glycerol using response surface methodology
Authors: Kumari, K Sita
Babu, I Sarat
Rao, G H
Keywords: Citric acid
Central composite design
Raw glycerol
Response surface methodology
Yarrowia lipolytica
Issue Date: Oct-2008
Publisher: CSIR
Abstract: Statistical experimental design was applied for the optimization of medium constituents for citric acid production by Yarrowia lipolytica NCIM 3589 in submerged fermentation using raw glycerol as the carbon source. Response surface methodology (RSM) involving central composite design (CCD) was adopted to evaluate the amount of citric acid produced by most important factors, such as carbon concentration, nitrogen concentration, and salt solution concentration. A second order polynomial regression model was fitted and was found adequate with R² of 0.9485. The optimum conditions were found to be carbon concentration 38.77 g/L, nitrogen concentration 0.401 g/L, and salt solution concentration 12.3 % (v/v). Citric acid production at these optimum conditions was 13.41 g/L.
Description: 496-501
URI: http://hdl.handle.net/123456789/2353
ISSN: 0972-5849
Appears in Collections:IJBT Vol.07(4) [October 2008]

Files in This Item:
File Description SizeFormat 
IJBT 7(4) 496-501.pdf501.98 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.