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Title: Genetic Diversity between Murrah and Bhadawari Breeds of Indian buffalo using RAPD-PCR
Authors: Barwar, Anurag
Sangwan, M L
Kumar, Sudhir
Ahlawat, Sonika
Keywords: RAPD
Buffalo
Characterization
Polymorphism
Genetic distance
Murrah
Bhadawari
Issue Date: Oct-2008
Publisher: CSIR
Abstract: Random amplified polymorphic DNA (RAPD) technique was used to characterize Murrah and Bhadawari breeds of buffalo. From the 9 random primers, a total of 84 bands were amplified between breeds and 51 of these (about 60.72%) were found to be polymorphic. In Murrah, overall percentage polymorphism of 56.36 was observed, while in Bhadawari it was 57.14. Higher genetic similarity of 0.81 and 0.80 within Murrah and Bhadawari breeds, respectively, was observed as compared to between breed genetic similarity of 0.31. Genetic distance between the breeds was 1.20. MAPD values of 10.72 and 16.10 were observed within Murrah and Bhadawari breeds, respectively, while MAPD of 60.73 was observed between breeds. Six primers (OPU-01, OPU-02, OPU-05, OPU-07, OPU-14 and OPV-14) in Murrah and five primers (OPU-05, OPU-07, OPU-14, OPU-19 and OPV-14) in Bhadawari were found to be specific for these breeds.
Page(s): 491-495
ISSN: 0972-5849
Source:IJBT Vol.07(4) [October 2008]

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