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Title: In vitro plant regeneration of red sanders (Pterocarpus santalinus L.f.) from cotyledonary nodes
Authors: Rajeswari, V
Paliwal, Kailash
Keywords: Cotyledonary node
ex-vitro rooting
Endangered species
Leaf nodes
Pterocarpus santalinus
Red sanders
Issue Date: Oct-2008
Publisher: CSIR
Abstract: A reliable and efficient micropropagation protocol was developed through axillary shoot proliferation from cotyledonary nodes of Pterocarpus santalinus. Cotyledonary nodes showed significantly (P<0.05) higher shoot multiplication rate and shoot length than leaf nodes on MS medium with 2.5 μM BAP and 2 μM 2-iP after first subculture (i.e. in the second harvest). For rooting, dipping of microshoots in 5 μM IAA solution proved superior to other in vitro methods. Of the various hardening media used for the acclimatization of rooted plants, a mixture of coarse sand, clay and farmyard manure (1:1:1) (v/v) supported the maximum percentage of survival (95%). There were no significant differences between the in vitro regenerated plants and seedlings of the same age for all the growth parameters measured (i.e. mean plant height, number of leaves per plant, fresh weight and dry weight of leaves, shoot and root per plant) four months after transfer to ex vitro conditions.
Page(s): 541-546
ISSN: 0972-5849
Source:IJBT Vol.07(4) [October 2008]

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