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Title: Efficient clonal propagation method for Decalepis hamiltonii, an endangered shrub, under the influence of phloroglucinol
Authors: Gururaj, H B
Giridhar, P
Ravishankar, G A
Keywords: Axillary bud;Clonal propagation;Decalepis hamiltonii;Multiple shoots;Phloroglucinol
Issue Date: Apr-2004
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl7 AO1H1/00
Abstract: A highly efficient two stage protocol was developed for induction of multiple shoots from single node in vitro shoot tip explants of Decalepis hamiltonii. It was found that phloroglucinol (PG) had synergistic effect on shoot multiplication when added with N6-benzyladenine and gibberellic acid. This protocol uses PG for both multiple shoot induction from nodal ex-plants, elongation of primary shoots and initiation of adventitious shoot formation from primary shoots, which was more in presence of triacontanol (TRIA). Maximum number of shoots per culture was observed on the medium containing N6 -benzyladenine (1.1μM ; BA), GA3 (5 .8 μM) and PG (800 μM ). Sub-culturing of the shoots onto MS medium containing optimum concentration of BA (5.6 μM ), PG (200 μM) and TRIA (0.011μM) produced elongated shoots along with secondary shoot formation. The long shoots were rooted on α-naphthalene acetic acid (5.38 μM; NAA) and PG (400 μM) containing medium. The rooted plantlets were hardened and their field survival rate was 80 -90%.
Page(s): 424-428
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.42(04) [April 2004]

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