Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/23382
Title: Alterations in radiation induced cell cycle perturbations by 2-deoxy-D-glucose in human tumor cell lines
Authors: Adhikari, J S
Dwarakanath, B S
Mathur, Rohit
Ravindranath, T
Keywords: Cell cycle;Cyclin;2-Dcoxy-D-glucose;G2/M checkpoint;Radiomodification
Issue Date: Dec-2003
Publisher: NISCAIR-CSIR, India
Abstract: In the present studies, effects of glucose analogue, 2-deoxy-D-glucose (2-DG) on radiation-induced cell cycle perturbations were investigated in human tumor cell lines. In unirradialed cells, the levels of cyclin B1 in G2 phase were significantly higher in both the glioma cell lines as compared to squamous carcinoma cells. Upon irradiation with Co60 gamma-rays (2 Gy), the cyclin B 1 levels were reduced in U87 cells, while no significant changes could be observed in other cell lines, which correlated well with the transient G2 delay observed under these conditions by the BrdU pulse chase measurements. 2-DG (5mM, 2 hr) induced accumulation or cells in the G2 phase and a time-dependent increase in the levels of cyclin B1 in both the glioma cell lines, while significant changes could not be observed in any of the squamous carcinoma cell lines. 2-DG enhanced the cyclin B1 level further in all the cell lines following irradiation, albeit to different extents. Interestingly, an increase in the unscheduled express ion of B1 levels in G1 phase 48 hr after irradiation was observed in all the cell lines investigated. 2-DG also increased the levels of cyclin D1 at 24 hr in BMG-1 cell line. These observations imply that 2-DG-induccd alterations in the cell cycle progression are partly responsible for its radioiomodifying effects.
Page(s): 1392-1399
URI: http://hdl.handle.net/123456789/23382
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(12) [December 2003]

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