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Title: Karyotype, Ag-NOR, CMA3 and SEM studies in a fish (Mystus tengara, Bagridae) with indication of female heterogamety
Authors: Das, Jayanta Kumar
Khuda-Bukhsh, A R
Keywords: Chromomycin A3;Chromosome;Female heterogamety;Karyotype;Myslus tengara;Nuclear organizer region
Issue Date: Jun-2003
Publisher: NISCAIR-CSIR, India
Abstract: Somatic karyotypes in M. tengara contained 54 chromosomes, comprising 26 homomorphic pairs in both sexes and one pair of heteromorphic nature in female (one big sub-metacentric and one small subtelocentric chromosomes), while in males this pair was homomorphic (with two big sub-metacentric chromosomes). The Nucleolus Organizer Regions (NORs) were located at one arm of the suspected sex elements in both sexes, while another pair of metacentric chromosomes (No.7) also showed Ag-positive arm. The CMA, technique revealed relatively bright fluorescing zones in the regions of chromosomes that showed Ag-positive staining, revealing thereby the preponderance of GC-rich active sites of rRNA genes in NORs. SEM studies revealed clear heteromorphism to exist in the elements suspected as sex chromosomes in females.
Page(s): 603-608
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(06) [June 2003]

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