Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/23297
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dc.contributor.authorEllaiah, P-
dc.contributor.authorPrakash, G Jaya-
dc.contributor.authorPrabhakar, T-
dc.contributor.authorSaisha, V-
dc.date.accessioned2013-11-12T04:38:12Z-
dc.date.available2013-11-12T04:38:12Z-
dc.date.issued2003-06-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/23297-
dc.description570-573en_US
dc.description.abstractA binary system for gene activation and site specific integration based on conditional recombination of transfected sequences mediated by FLP recombinase from yeast was implemented in mammalian cells. In several cell lines, FLP rapidly and precisely recombined copies of its specific target sequences to activate an otherwise silent beta-galactosidase reporter gene. Clones of marked cells were generated by excisional recombination within a chromosomally integrated copy of the silent reporters. These clones exhibited intense blue colour with X-Gal staining solution. en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.41(06) [June 2003]en_US
dc.subjectBHK-2l cell lineen_US
dc.subjectFLP-recombinaseen_US
dc.subjectRecombinaseen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.titleSite specific integration of FLP recombinase in BHK-21 cell lineen_US
dc.typeArticleen_US
Appears in Collections:IJEB Vol.41(06) [June 2003]

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