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Title: Cloning and sequencing of 28 kDa outer membrane protein gene of Brucella melitensis Rev. 1
Authors: Chaudhuri, Pallab
Kumar, S Vinoth
Prasad, Rajeev
Srivastava, S K
Yadav, M P
Keywords: Brucella melitensis;Cloning;Outer membrane protein
Issue Date: Sep-2005
Publisher: NISCAIR-CSIR, India
Abstract: Brucella melitensis is an organism of paramount zoonotic importance. The 28 kDa outer membrane protein (OMP) is one of the immunodominant antigens of B. melitensis. The gene encoding 28 kDa OMP (omp28) has been amplified from B. melitensis Rev. 1 strain. A PCR product of 753 bp, encoding complete omp28 gene of B. melitensis, was obtained. The gene was further cloned and sequenced. The nucleotide sequence of B. melitensis Rev.1 strain showed substitution of 2 nucleotides from that of 16M strain.
Page(s): 838-840
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.43(09) [September 2005]

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