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Title: Enhanced proteolysis leads to pre-mature cell death under the influence of elicitor like mycelial components from Karnal bunt (Tilletia indica) pathogen in wheat callus cultures
Authors: Mani, Shalini
Yadav, Manoj K
Khan, Gohar Taj
Singh, U S
Kumar, Anil
Keywords: Elicitor;Kamal bunt;Programmed cell death;Proteolysis;Wheat callus
Issue Date: Aug-2005
Publisher: NISCAIR-CSIR, India
Abstract: Calli raised from mature embryos of susceptible wheat cultivar WH 542 were used in the present study as in vitro bioassay system to study the influence of disease determinant(s) of Karnal bunt (Tilletia  indica), a semi-biotrophic fungal pathogen of wheat. Influence of elicitor and conditioned medium (CM) prepared from fungal cultures of T. indica was investigated on induction of programmed cell death (PCD). Induction of PCD was observed as hypersensitive response (HR) in terms of browning at localized regions of call us cultures and induction of proteolytic enzyme(s). Elicitor treated calli showed higher induction of protease activity than untreated and CM-treated cultures, which showed not much change in the activity. It was further substantiated by gel protease assay and activation of caspase-3 like protein(s) in call us cultures that clearly suggested the presence of signaling molecule(s) in the fungal elicitor preparation rather than in conditioned medium. This study further demonstrated that only elicitor preparation possesses such molecule(s), which might be cell wall bound components, rather than secretory in nature as CM was unable to induce PCD in wheat callus cultivars.
Page(s): 746-750
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.43(08) [August 2005]

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