Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/23035
Title: Inhibitory effect of sildenafil on gastrointestinal smooth muscle: Role of NO-cGMP transduction pathway
Authors: Patil, Chandrashekhar S
Singh, Vijay Pal
Jain, Naveen K
Kulkarni, Shrinivas K
Keywords: Gastric emptying;Intestinal motility;Non-adrenergic non-cholinergic transmission;Sildenafil
Issue Date: Feb-2005
Publisher: NISCAIR-CSIR, India
Abstract: Nitric oxide (NO) is an important neurotransmitter in the gut and has been demonstrated to be a key physiological mediator of non-adrenergic non-cholinergic (NANC) relaxation of gastrointestinal smooth muscle. In the present study the effect of PDE 5 inhibitor sildenafil on the gastrointestinal function (gastric emptying and intestinal transit) has been demonstrated in mice. Sildenafil (0.5-2 mg/kg, po) did not alter the percent gastric emptying however, in higher doses (5,10 and 30 mg/kg, po) it inhibited the gastric emptying. On acute administration (0.5-5 mg/kg, po) it did not alter the intestinal transit but in higher doses (10 and 30 mg/kg, p.o.) delayed the intestinal transit. Further, the inhibitory effect of sildenafil was significantly blocked by L-NAME (10 mg/kg, ip), a non-selective NOS inhibitor and methylene blue (1 mg/kg, ip ), a guanylate cyclase inhibitor. These findings suggest the participation of NO-cGMP transduction pathway in the inhibitory effect of sildenafil (higher doses) on the gastrointestinal smooth muscles and its potential application in patients with nutcracker oesophagus, hypertensive lower oesophageal sphincter (LOS), achalsia and diabetic gastroparesis or colitis where there is a loss of nNOS.
Page(s): 167-171
URI: http://hdl.handle.net/123456789/23035
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.43(02) [February 2005]

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