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dc.contributor.authorMukunthakumar, S-
dc.contributor.authorSeeni, S-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.description.abstractTwo primitive diploid Musa cultivars, Matti and Chemmatti from the extreme southern part of the Western Ghats were multiplied by in vitro culture of sucker-derived shoot apices. Decontaminated corm ex plants (1cm×1cm) having shoot apex (~ 0.3 cm) cultured for l month in Murashige and Skoog basal agar medium was cut vertically into eight segments and each segment having a part of shoot meristem was cultured in presence of 6- benzylaminopurine (BAP) and combinations of BAP and indole-3-acetic acid (IAA) or indole-3-butyricacid (IBA) to produce multiple shoots. After 12 weeks of culture, maximum number of shoots (32) in both the cultivars were produced in approximate 60% of the explants in presence of BAP and IAA each at 1.5 mg/l-1 (Matti) and 40% of the explants in 2.5 mg/l-1 of BAP and 1.5 mg/l-1 of IAA (Chemmatti). Buds were formed from the base of the subcultured shoots and somewhat more number (34) of shoots were obtained in Matti than in Chemmatti (31) after 8 weeks. Difference in the concentration of cytokinin required for shoot initiation and multiplication, persistence of exudation through the subculture and red colouration of the early formed sheathing leaf bases in the shoots in Chemmatti indicated possible genotypic differences between the two cultivars. Multiple shoot proliferation achieved through five subcultures of the isolated shoots without any decline. Transfer of shoots (4-5 cm) into MS basal medium favoured rooting in 4 weeks and rooted plants (9 cm) were hardened and established (80-95%). Mericlones of Matti cultivated in homesteads produced bunches of uniform characters in 13 months.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.43(01) [January 2005]en_US
dc.subjectHomestead cultivationen_US
dc.subjectNative diploidsen_US
dc.titleIn vitro cloning and homestead cultivation of primitive Musa cultivarsen_US
Appears in Collections:IJEB Vol.43(01) [January 2005]

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