Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/21849
Title: Micropropagation of Stemona tuberosa Lour.—An endangered and rare medicinal plant in Eastern Ghats of India
Authors: Murthy, K Sri Rama
Reddy, M Chandrasekhara
Kondamudi, R
Pullaiah, T
Keywords: Micropropagation;Multiple shoots;Stemona tuberosa;Tuberous roots
Issue Date: Jul-2013
Publisher: NISCAIR-CSIR, India
Abstract: An in vitro technique of multiple plantlet regeneration was developed for conservation of endangered wild medicinal plant Stemona tuberosa Lour.. Murashige and Skoog (MS) medium supplemented with 7.0 mg/L Kn (kinetin) was found to be the optimum for axillary bud proliferation and multiple shoot induction. Excision and culture of nodal segments from the in vitro-shoots on medium containing 7.0 mg/L Kn and 4.0 mg/L TDZ (thidiazuron) showed maximum number of shoot multiplication with 7.10±0.37 shoots/node. Rapid shoot growth with simultaneous tuberous root formation was also observed in the same concentration. Shoots developed were rooted best on ½ strength MS with 1.0 mg/L IAA (indole acetic acid). Plantlets established in pots exhibited 85% survival. Plantlets successfully established in field exhibited morphological characters identical to mother plants.
Page(s): 420-424
URI: http://hdl.handle.net/123456789/21849
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.12(3) [July 2013]

Files in This Item:
File Description SizeFormat 
IJBT 12(3) 420-424.pdf121.77 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.