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Title: Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars
Authors: Mariashibu, Thankaraj Salammal
Subramanyam, Kondeti
Arun, Muthukrishnan
Theboral, Jeevaraj
Rajesh, Manoharan
Rengan, Sampath Kasthuri
Chakravarthy, Rajan
Manickavasagam, Markandan
Ganapathi, Andy
Keywords: Amino acids;Genotype;Immature cotyledon;Somatic embryogenesis;Soybean
Issue Date: Oct-2013
Publisher: NISCAIR-CSIR, India
Abstract: Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded were higher with 45.24 µM 2,4-D. The proliferation of somatic embryos for three successive cycles was achieved in 22.62 µM 2,4-D. Histodifferentiation of somatic embryos under NAA (10.74 µM) indicated that better embryo development and maturation was achieved without any growth regulator. The amino acids such as L-glutamine favoured the somatic embryo induction and histodifferentiation at 20 and 30 mM respectively, where as L-asparagine at 10 mM concentration enhanced the somatic embryo proliferation. In addition, somatic embryos that were desiccated (air-drying method) for 5 days showed better germination (40.88%). The Indian soybean cultivars also showed strict genotypic influence and cv. Pusa 16 was emerged as a best responding cultivar for somatic embryo induction with 74.42% of response.
Page(s): 849-859
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.51(10) [October 2013]

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