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Title: Isolation and characterization of meta-toluic acid degrading marine bacterium
Authors: Prakash, Divya
Raushan, Rakesh Kumar
Sangodkar, U.M.X
Rivonker, C.U.
Keywords: Bio-degradation;Marine pollution control;Substrate constant (Ks);Michaelis constants (Km) values;Crude oil;Tar balls;Catechol 2, 3 dioxygenase and hazardous chemicals
Issue Date: Sep-2008
Publisher: CSIR
Abstract: The analysis of the sea water samples using sequential enrichment technique revealed a report of marine bacterium capable of degrading meta toluic acid a component of crude oil. An attempt to characterize the isolated culture using biochemical tests indicated the culture as a Gram- negative aerobic rod that was highly motile exhibiting biodegrading ability and was identified as Pseudomonas spp. strain GUI13. Further, a comparative analysis of the biochemical characters with the archae type terrestrial soil bacterium indicated that the isolate required marked amounts of Sodium chloride (NaCl) in the medium to retain its viability. Substrate constant (Ks) of strain GUI13 with respect to meta toluic acid was found to be eight times lower when compared to that of a terrestrial bacterium. A similar ratio was observed in case of Michaelis constants (Km) for the key degradative enzyme, Catechol 2,3 dioxygenase, emphasizing the distinguishing feature of the marine bacteria that helps it to carry on the process of bio transformations at very low concentrations of carbon, a unique condition that exists in the sea.
Page(s): 322-325
ISSN: 0379-5136
Appears in Collections:IJMS Vol.37(3) [September 2008]

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