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Title: Fabrication of biotin functionalised SiO2 EM grid for studying biotin tagged biomolecules
Authors: Chakraborty, Indrani
Saha, Abhijit
Ghosh, Surajit
Keywords: Electron microscopy;Surface functionalisation;Silica EM grid;Gold nanoparticles;Nanoparticles;Microtubules;Biotin
Issue Date: Aug-2013
Publisher: NISCAIR-CSIR, India
Abstract: A biotin functionalised SiO2 electron microscope grid has been developed for studying various biotin/avidin tagged biomolecules onto the grid surface under electron microscope. As an application, we have shown successful immobilisation of streptavidin gold nanoparticles and biotinylated microtubules through biotin-streptavidin-biotin interaction. This grid will be useful for studying the structures of various biotin-tagged proteins and their complexes under electron microscope.
Page(s): 1026-1030
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.52A(08-09) [August-September 2013]

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