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|Title:||Fabrication of biotin functionalised SiO2 EM grid for studying biotin tagged biomolecules|
|Keywords:||Electron microscopy;Surface functionalisation;Silica EM grid;Gold nanoparticles;Nanoparticles;Microtubules;Biotin|
|Abstract:||A biotin functionalised SiO2 electron microscope grid has been developed for studying various biotin/avidin tagged biomolecules onto the grid surface under electron microscope. As an application, we have shown successful immobilisation of streptavidin gold nanoparticles and biotinylated microtubules through biotin-streptavidin-biotin interaction. This grid will be useful for studying the structures of various biotin-tagged proteins and their complexes under electron microscope.|
|Appears in Collections:||IJC-A Vol.52A(08-09) [August-September 2013]|
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|IJCA 52A(8-9) 1026-1030.pdf||145.94 kB||Adobe PDF||View/Open|
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