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Title: Rapid synthetic access to caged RNA
Authors: Rodrigues-Correia, Alexandre
Stoess, Tatjana
Wilhelm, Julia
Berlin, Beata
Heckel, Alexander
Keywords: RNA;Caged RNA;Light-activatable RNA;Duplex destabilizing
Issue Date: Aug-2013
Publisher: NISCAIR-CSIR, India
Abstract: Light-activatable RNA with “caged” nucleobases is a versatile tool for light-control of RNA interference, riboswitch folding or miRNA or ribozyme action. Till date the respective caged and protected phosphoramidites for the solid-phase RNA synthesis are obtained in lengthy syntheses. Herein we explore the use of convertible nucleoside residues for a rapid access to nucleobase-caged RNA. In these studies we have also prepared the first RNA with a caging group on an N2-position of a guanosine. The obtained caged sequences are compared in their duplex destabilizing ability via melting temperature measurements.
Page(s): 1014-1018
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.52A(08-09) [August-September 2013]

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